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Slideshow 226386 by Lucy. PowerPoint Presentation - CARDIOVASCULAR PATHOLOGY 1. CARDIOVASCULAR PATHOLOGY Tutorial I William H. It is important for students to recognize discrepant results and how to. Correct interpretation of panel studies requires PRACTICE. The following case studies will illustrate a systematic method of evaluating and. REMEMBER, antigens are present on the RED. BLOOD CELLS. A positive reaction with a cell indicates the presence of an ANTIBODY. ANTIGEN present on the reagent cells. An excellent overview of the process is illustrated in a Power. Point presentation. Renee Wilkins, Ph. D, MLS(ASCP)cm from the University of Mississippi. Medical Center: Antibody Identification. Crucial to the investigation of the antibody is the patient's history: Has the patient ever been pregnant? Pregnancy can cause immunization of. LACKS and which. was inherited from the father. Has the patient ever been transfused? Index(Continued): Index(Continued) Basic Enzymology Labels Antigen-antibody binding reaction Direct Method Indirect Method Antigen Retrieval Techniques Blocking. Investigation strategies and methods Antigen and antibody detection May 2007 Learning objectives At the end of the presentation, participants should Understand direct. Enzyme Linked Immunosorbent Assay (ELISA) ELISA Enzyme Linked Immunosorbent Assay (ELISA) Term Was Coined By Engvall and Pearlmann in 1971 Different Types Sandwich. Antigen ab reactions 1. Antigen-Antibody Interactions: 2. Quantifying Antigen-Antibody reactions <ul><li>Ag-Ab interaction is a. Transfusion exposes the patient to. RBC antigens they do not possess and can cause immunization to antigens they. RBCs. What is the patient's diagnosis? This may offer clues as to the patient's. RBC antigens. Perhaps the patient has sickle cell anemia, a frequently. Highlight. the panel, click on . If you CANNOT correctly identify the specificity. Carefully review the tutorial for selecting positive. Last Update: March. Web Author: Terry Kotrla, MS, MT(ASCP)BBComments: kotrla@austincc. Immunohistochemistry Seminar 5 . Bhale. Rao (Professor) Dr. Sandhya Tamgadge (Professor) Dr. D. Y. Patil Dental College Oral Pathology & Microbiology. Index: Index Basic immunohistochemistry steps Definition Antigens Ig. G antibodies Monoclonal antibodies formation Polyclonal antibodies formation Handling of antibodies. Index(Continued): Index(Continued) Basic Enzymology Labels Antigen- antibody binding reaction Direct Method Indirect Method Antigen Retrieval Techniques Blocking Endogenous Enzymes Blocking Background Staining. Power. Point Presentation: IMMUNOHISTOCHEMISTRY STEPS Slide 3 of 2. Tissue sections Antigen retrieval Blocking endogenous enzymes Secondary antibody Primary antibody Microscopy Observation Blocking background staining Chromogen Substrate Counterstain Mounting. Antigen retrieval: Antigen retrieval Routine histology procedures masks the antigens, usually by formalin cross- linking, or even destroys some antigen epitopes. So to retrieve this antigens we use retrieval procedures. Blocking endogenous enzymes: Blocking endogenous enzymes Most popular enzymes used today are trypsin and protease. Because digestion breaks down formalin cross- linking and hence the antigenic sites for a number of antibodies are uncovered. Impurities in crude trypsin such as chymotrypsin are the active ingredients. Essential that commercial companies constantly monitor & standardise chymotrypsin levels in the batches of trypsin they supply in order to assist users with the reproducility of their technique. Enzymes similar to those used as tracer are present in tissue, they may react with the substrate used to localize the tracer and give rise to problems in interpretation. Contd.: Contd. Peroxidase and substances giving a pseudo- peroxidase reaction are present in some normal and neoplastic tissues i. Endogenous peroxidases are first localised by using one chromogen and then following immunocytochemical staining, the enzyme tracer is localized by an alternative chromogen which yields a reaction end- product in contrasting color. Blocking background staining: Blocking background staining Non- specific uptake of antigen, particularly high affinity of collagen and reticulin for immunoglobulins can cause high levels of background staining. Specific background staining also occurs when primary antibody is contaminated with other antibodies. Non- specific background staining is non- immunological binding of the specific immune sera by hydrophobic and electrostatic forces to certain sites within tissue sections. A. H. Coons: A. H. Coons In 1. 94. 0, (Microbiologist) described identification of tissue antigens in pneumococci by using direct fluorescence. Definitions: Definitions Immunohistochemistry is a technique for identifying tissue constituents (antigens) by means of antigen- antibody interactions. The site of antibody binding being identified either by direct labeling of the antibody, or by use of secondary antibody labeling method . Is Immunohistochemistry(IHC) and Immunocytochemistry(ICC) one and the same?: Is Immunohistochemistry(IHC) and Immunocytochemistry(ICC) one and the same? Mobility of Antigens: Mobility of Antigens Ig. G Antibodies: Ig. G Antibodies VL and VH together form the antigen- combining site. The H chain also has three constant domains (CH1, CH2,CH3) and carries the carboxyl terminal portion of the immunoglobulin. Power. Point Presentation: Digestion by papain results in the cleavage of a susceptible bond on the N- terminal side of the inter- heavy chain disulfide bridges . Power. Point Presentation: Pepsin cleaves the gamma chains on the C- terminal side of the inter- heavy chain disulfide bridges, resulting in one bivalent antigen- binding fragment F(ab. Properly maintained, most reagents will remain stable for months and even years. The recommendations given by the manufacturer on specification sheets and on vial labels should always be followed. Receiving of antibodies: Receiving Although most commercially produced immunochemicals are guaranteed to be stable for up to several years, reasonable precautions taken by the user will help maintain this stability. Upon receipt, immunochemicals should be promptly stored according to the manufacturer. The reagents should be logged in by entering the manufacturer. These entries provide valuable information for the user, especially if later reclamations become necessary. Receiving of antibodies. Storage of antibodies: Storage of antibodies Storage Perhaps the two most important considerations when storing antibodies are the storage container and the temperature. Storage containers The preferred materials for storage containers of protein solutions should have negligible protein absorptivity. Polypropylene, polycarbonate or borosilicate glass are particularly recommended and are used widely. Protein concentration: Solutions containing very low concentrations of protein(i. Generally 0. 1% to 1. Mustek Bear. Paw 4. TA Pro II Scanner Drivers Download and Update for Windows 1. XP and Vista. Are you looking for Mustek Bear. Paw 4. 80. 0TA Pro II Scanner drivers? Just view this page, you can through the table list download Mustek Bear. Paw 4. 80. 0TA Pro II Scanner drivers for Windows 1. Vista and XP you want. Here you can update Mustek drivers and other drivers. Driver DR is a professional Windows drivers download site, it supplies all devices for Mustek and other manufacturers. 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